Resolution of the Spatial Distribution of the Bacteriophage MS2 Protein and RNA Components

Abstract

Small-angle neutron scattering (SANS) and the contrast variation technique were used to determine the spatial relationship of the individual components of the MS2 virion (protein shell and genomic RNA). Our results show that (a) the MS2 RNA is tightly compacted within the virion, confined to a radius of 83 +/- lA and (0) the MS2 coat protein shell extends to a radius of 136 + 2A and has a thickness of 2l +/- lA. Therefore, the protein core is composed primarily of solvent, and the RNA core is compact.

Open PDF

Document Details

Document Type
Technical Report
Publication Date
May 01, 2005
Accession Number
ADA452166

Entities

People

  • Catherine O'connell
  • Charles H. Wick
  • Deborah A. Kuzmanovic
  • Iiya Elashvili
  • Susan Krueger

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Bacteriophages
  • Chemistry
  • Detectors
  • Iridoviridae
  • Leviviridae
  • Microbiology
  • Molecular Biology
  • Neutron Scattering
  • Physical Properties
  • Ribonucleic Acids
  • Rna Phages
  • Rna Viruses
  • Scattering
  • Spatial Distribution
  • Three Dimensional
  • Viral Structures
  • Viruses

Readers

  • Astronomy/Astrophysics
  • Mechanical Engineering/Mechanics of Materials.
  • Molecular Genetics