Characterization of Steroid Receptor RNA Activator Protein Function in Modulating the Estrogen Signaling Pathway
Abstract
The core of my hypothesis is that Steroid receptor RNA activator protein, a newly discovered protein from a once thought noncoding RNA, is involved in the modulation of the estrogen signaling pathway. The steroid receptor RNA activator (SRA) differs from all previously characterized co-activators as it is currently the first molecule potentially active at both the RNA and protein levels. Today, all functional studies on the human SRA concentrate on its RNA function and ignore the existence of the protein. We therefore believe there is an urgent need to analyze the function of the SRA protein (SRAP). The aim of the first year of the project was to first determine if SRAP affects the expression of ER target genes such as pS2 and PR. Additional aims for the first year were also to determine the effect of SRAP expression and the growth rate and invasive properties of MCF-7 cells over-expressing SRAP. In the first year, I have shown that the expression of pS2 is unaffected by SRAP. However PR is differentially expressed in the cells over-expressing SRAP. I have also determined that MCF-7 cell lines stably expressing SRAP are not a suitable model to analyze proliferation and invasion differences. In the interim, I have successfully generated the plasmids necessary to investigate SRA RNA and protein function independently. Through mass spectrometric analysis of proteins co-immunoprecipitated with SRAP, I have identified 69 possible SRAP interacting proteins.
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 01, 2006
- Accession Number
- ADA452444
Entities
People
- Chooniedass Shilpa
Organizations
- University of Manitoba