Functional Characterization of Two Novel Human Prostate Cancer Metastasis Related Genes
Abstract
We propose to identify the functional characterization of two novel cancer-specific, metastasis-related genes whose constitutive expression may be pivotal for prostate cancer progression. Work accomplished in the first year was performed based on the proposed statement of work. We have characterized the full-length cDNAs of the Seq1 and Seq2 genes using at least three 5 and 3 rapid amplification of cDNA ends (RACE) commercial kits (Invitrogen Carlsbad, CA, BD Bioscience (Clontech Inc), and Seegene, Rockville, MD). In the second year, we subcloned cDNAs in expression plasmids and were sequence verified for orientation and recombinant protein expression, in vitro translation, and antibody production. Our research plans of development of antibodies and validation of expression in tissue sections were disrupted by hurricane Katrina and destruction of all of our laboratory materials and supplies and experimental animals. Tulane is now working hard towards restructuring our facilities to resume our research project in the Spring/2006.
Document Details
- Document Type
- Technical Report
- Publication Date
- Feb 01, 2006
- Accession Number
- ADA452468
Entities
People
- Asim B. Abdel-mageed
- Byron Crawford Jr.
- Krishna C. Agrawal
Organizations
- Tulane University of Louisiana