Characterization of a Dopaminergic Stimulatory Factor Derived From Monoclonal Cell Lines of Striatal Origin
Abstract
A lysate of an immortalized monoclonal cell line derived from the striatum (X61) contains an activity that is capable of increasing the dopamine content of an immortalized, dopaminergic mouse mesencephalic cell line (MN9D). The activity can be extracted from the cell lysate by isoamyl alcohol/chloroform. NMR spectroscopic analysis demonstrated that a purified fraction from the isoamyl alcohol/chloroform soluble dopaminergic stimulatory activity is a mixture of 80-90% cis-9- octadecenoic acid (oleic acid) and 10-20% cis-11-octadecenoic acid (cis-vaccenic acid). MN9D dopamine increases linearly in the presence of oleic acid over a 48 hour period suggesting the induction of an increased dopaminergic phenotype in these dividing cells. The ability to increase MN9D dopamine by oleic and cis-vaccenic acids is shared by a number of other longchain fatty acids including arachidonic, linoleic, linolenic, palmitoleic, and cis-13-octadecenoic acid. Studies on the mechanism of elevation of MN9D dopamine by oleic acid suggest that this fatty acid increases both the synthesis and uptake and/or storage of dopamine. The possibility that oleic or other relatively innocuous fatty acids might affect dopaminergic function in primary neurons is intriguing with respect to possible therapeutic approaches to the treatment of dopaminergic cell loss and the motor sequelae of Parkinson's disease.
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 01, 2005
- Accession Number
- ADA452472
Entities
People
- Alfred Heller
Organizations
- University of Chicago