Origin and Properties of Prostatic Stem Cells
Abstract
The aim of this proposal is to isolate and characterize prostatic epithelial stem cells. This will be done as stem cell and tumor cell biology may be closely linked and stem cells may have a role in the etiology of cancer. Our results show that the prostate contains a small population of cells (<1%) capable of effluxing the Hoechst dye by an active process(the side population, SP), indicating that cells with features of stem cells are found in the prostate. As 96% of the SP cells express Sca-1, a protein expressed by stem cells of other origins, we isolated Sca-1 expressing cells and assayed their proliferative ability in an in vivo prostate reconstitution assay. These results show that prostate regenerating ability resides in the Sca-1 expressing population. In addition, we show that prostate stem cells reside in the proximal region of ducts within the population that expresses high levels of Sca-1. We also show that bone marrow cells can differentiate into prostatic epithelial(basal) cells and fibroblasts with significantly more fibroblasts (7%) being of bone marrow origin than basal epithelial cells (1%).
Document Details
- Document Type
- Technical Report
- Publication Date
- Feb 01, 2006
- Accession Number
- ADA452535
Entities
People
- E. L. Wilson
- Rashmi Gupta
Organizations
- New York University