Toxicity of Experimental Jet Fuel System Ice-Inhibiting Agents: I. In Vitro Dosimetry
Abstract
The potential toxicities of compounds to replace current US Air Force operational chemicals is of particular interest. A group of jet fuel system ice-inhibiting agent alternatives currently being evaluated for performance include derivatives of 1,3-dioxolane-4-methanols and 1,3-dioxanes. The experiments presented here were performed in support of Air Force Research Laboratory's Materials Directorate research on FSII alternatives. Our initial approach to evaluating potential toxicity utilized primary rat hepatocyte cultures for assessing acute toxicity. The experiments summarized here were performed to develop analytical methods for these chemicals and the assessment of potential variations in the aqueous concentrations of the test chemicals during cell culture experiments due to volatilization. The test chemicals were prepared at lmM and lOmM concentrations and incubated in 6-well culture plates at 37 C for 0-6 hrs. Aliquots were taken at time intervals and analyzed by gas chromatography. These concentrations indicate the potential in vitro dosimetry for the cells in culture. The results indicate that for all but one (M-27) for M-DE and M-PE there was no significant change in chemical concentration of the chemicals tested, the aqueous concentrations do not vary substantially from their initial concentration over a six hour incubation.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 1999
- Accession Number
- ADA453145
Entities
People
- Dan L. Polland
- John M. Frazier
- Kevin T. Geiss