Evaluation of DNA Methylation as a Target for Intraductal Therapy for Ductal Carcinoma in Situ of the Breast
Abstract
Ductal carcinoma in situ(DCIS), the preinvasive form of infiltrating ductal breast cancer, accounts for 20-30% of breast cancers and is treated surgically. In DCIS, the malignant cells are confined within the basement membrane, and so an ideal candidate for local therapies. Because DNA methylation is a potentially reversible mechanism for tumor suppressor gene inactivation, it is an intriguing target for molecular therapeutics. Hypothesis: DNA Methylation is altered in DCIS and is a therapeutic target for intraductal therapy. Specific Aim 1: Document the methylation status of tumor suppressor genes in DCIS. We have documented significant methylation in eight tumor suppressor genes in DCIS. Specific Aim 2: Document the feasibility of an intraductal approach to DCIS. We have successfully performed ductal lavage in 5 patients undergoing surgery for DCIS without any complications. Specific Aim 3: Identify the dose(s) of DAC with biologic activity and acceptable side effects when delivered intraductally to patients with DCIS (Phase I trial). The ultimate goal of this proposal is to evaluate DNA methylation as a target for intraductal therapy.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 2005
- Accession Number
- ADA453373
Entities
People
- Kristin A. Skinner
Organizations
- New York University