Development and Testing of a Novel Standard Particle for Performance Verification of Biodefense/Bioterrorism Detection Systems

Abstract

This paper describes a novel simulant test particle that allows for the calibration of biodetection systems without causing safety concerns. Specifically, this test particle is a polystyrene sphere, of an aerodynamic diameter similar to that of Bacillus anthracis, with Bacillus globigii genomic DNA attached to the surface. The DNA attachment is strong enough to withstand aerosolization and collection by a SpinCon sampler, and yet weak enough to detach during the GeneXpert analysis. Tests performed at MRI have shown that the tagged beads can be collected in the SpinCon and then successfully analyzed in the GeneXpert without modifying currently established analysis protocols.

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Document Details

Document Type
Technical Report
Publication Date
Nov 19, 2003
Accession Number
ADA453904

Entities

People

  • Andrew E. Page
  • David S. Alburty
  • Freeman J. Swank
  • Jennifer L. Dannehl
  • Kelly L. Brown
  • Mike F. Fischer
  • Robert C. Huebner

Organizations

  • MRIGlobal

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Abstracts
  • Attachment
  • Biodefense
  • Biological Aerosols
  • Bioterrorism
  • Boiling Point
  • Chemical Reactions
  • Detection
  • Dielectric Polymers
  • Fungi
  • Materials
  • Particles
  • Polymerase Chain Reaction
  • Polystyrenes
  • Propellants
  • Standards
  • Vapor Pressure

Fields of Study

  • Physics

Readers

  • Aerosol Science/Aerosol Physics
  • Military/Explosive Ordnance Disposal (EOD) Technology
  • Systems Analysis and Design