Activation and Protection of Dendritic Cells in the Prostate Cancer Environment
Abstract
First annual report for this award. Experiments were conducted as was scheduled in the Statement of Work. So far studies have demonstrated for the first time the presence of endothelin receptors on murine DC, and the fact of endothelin-1 production by murine DC upon stimulation with TNF+. Phenotyping of dendritic cells stimulated with TNF+ and treated with endothelin receptor inhibitors demonstrated decreased expression of pro-inflammatory co-stimulatory molecules (CD4O, VD8O, CD86, CD205, MHC class II) with the blockade of ETA receptors, and no change or mild increase in the expression of co- stimulatory molecules with the blockade of ETB receptors. In vivo administration of endothelin A receptor inhibitor abolished the effect of infectious stimulus to mobilize dendritic cells to draining lymph nodes. Functional studies are under way to further characterize the role of endothelin receptors in the biology of dendritic cells, as well as to study the interaction of dendritic cells and prostate cancer cells, and develop the means of active cell therapy for murine prostate cancer model.
Document Details
- Document Type
- Technical Report
- Publication Date
- Feb 01, 2006
- Accession Number
- ADA454002
Entities
People
- Georgi Guruli
- Mark L. Jordon
Organizations
- University of Medicine and Dentistry of New Jersey