Novel Role of Candidate Tumor Suppressor ANX7 Gene in Prostate Cancer
Abstract
The purpose of this study is to determine the mechanism and the signaling pathway by which the ANX7 gene induces death of prostate cancer cells. The ANX7 induced apoptotic pathway involves calcium and cytochrome c release and morphological changes, nuclear fragmentation and chromatin condensation. Overexpression of ANX7 reduces the percentage of cells that are capable of responding to the IP3-generating agonist acetylcholine with a reduction in the magnitude of the response to acetylcholine. Using cDNA microarray and Western blot analysis, we have identified the downstream targets and signaling pathway of ANX7 in apoptosis and suppression of prostate caner cell growth. We identified the apoptosis, metastatic and cell cycle corrected genes which show equivalent relative expression in PREC normal prostate cells and transfected metastatic DU145 cancer cells with ANX7J, wt-ANX7 or P53. Taken together, these data indicate that ANX7 suppresses prostate cancer cell growth by apoptosis and calcium plays a role via IP3-Receptor. In addition, we have identified the members of the members of the ANX7 signaling pathway. ANX7, Calcium, IP3 Receptor, Signal Transduction, cDNA Microarray
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 01, 2005
- Accession Number
- ADA454004
Entities
People
- Meera Srivastava
Organizations
- Henry M. Jackson Foundation for the Advancement of Military Medicine