Positional Cloning of an Ashkenzai Jewish Hereditary Prostate Cancer
Abstract
The fellowship aimed to confirm the 7q11-21 linkage result, to identify the founder haplotype and to clone the disease- associated mutation. The final research accomplished has: 1) confirmed the 7q11-21 linkage result by analysis of additional microsatellite markers in the 37.6 cM region and a combined genome-wide scan of 36 Jewish HPC families (empirical P=O.006); 2) defined the minimal recombination region for the 18 PROGRESS Jewish families as a 5.7 cM interval with 21 RefSeq genes; 3) sequenced almost all of the exons in the MRR (1191134, 88%); 4) genotyped key individuals from the 18 Jewish families on the Affymetrix 1OOK SNP chips (163 total chips); 5) built the chromosome 7 haplotypes in each family with the 7,069 SNPs from the SNP chips; 6) designed and wrote analysis scripts to identify haplotype patterns enriched in the affecteds; 7) investigated 22 regions further by genotyping 113 potentially informative SNPs from the HapMap project; 8) generated a large volume of chromosome 7 genetic information with a total of 73 microsatellites, 7,069 chip SN Ps, and 727 sequenced SNPs or amplicons with one or more exons.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 2006
- Accession Number
- ADA454005
Entities
People
- Danielle Friedrichsen
Organizations
- Fred Hutchinson Cancer Center