An Imaging System to Monitor Efficacy of Adenovirus-Based Virotherapy Agents
Abstract
Our preliminary data establish a number of important key points. Foremost, these results show that adenovirus can be genetically labeled with a fluorescent structural fusion protein through a complete replacement with IX-EGFP in a chimeric context. At least for our pIX-EGFP strategy, the label was incorporated into virions conferring a fluorescent property that allowed detection of individual particles. Ad-IX-EGFP binding and infection could both be detected via the fluorescent label. This capsid-labeling system is applicable to CRAds because it slightly decreased progeny yield but did not affect the cytopathic effect and spread of the virus. Notably, the level of pIX-EGFP fluorescence directly correlated with the amount of progeny production due to its dependence on E1 activity for expression. The data with pIX-EGFP fulfills all the requirements of the ideal monitoring system for CRAds except noninvasive detection which we propose to accomplish. Both our proposed capsid-labeling approaches demonstrate great promise for detection of viral replication and spread and hence monitoring of CRAds.
Document Details
- Document Type
- Technical Report
- Publication Date
- Feb 01, 2006
- Accession Number
- ADA454309
Entities
People
- David T. Curiel
Organizations
- University of Alabama