Prediction of Aggressive Human Prostate Cancer by Cathepsin B
Abstract
I received approval of the DOD-HSRRB (Log No. A-12517) around 20th April, 2005 and began the study without further delay. We conducted portions of task 1, 2, 3, 5 and 6 studies on radical prostatectomy (RP) and biopsy tissue samples and collected clinical data from the same white and African American men. The Elisa assay, part of task 4, laser capture microdisection (LCM) in this task is in progress. Prostate cancer (PC), and benign prostatic hyperplasia (BPH) as control samples, were collected at the VA Medical Center and Virginia Urology Center. Our study has resulted in two manuscripts, 1. Prognostic Value of the Cathepsin B to Stefin A Ratio in Prostate Needle Biopsies and 2. Characterization of Prostate Cancer in African American Men by Cathepsin B and Stefin A. Since all co-authors have not reviewed the manuscripts, we are enclosing drafts prior to their submission to peer-reviewed journals. Study 1. Our objective was to define characteristics of cancer cells in low volume (about 10% in 1 or 2 biopsy cores) and high volume (about 50% in 1 or 2 cores) Gleason score 6 tumor biopsies using cathepsin B (CB) and stefin A (SA), immunohistochemistry (IHC) and quantitative image analysis. We evaluated biopsies with the Gleason score 6 tumors in 65 patients and post-RP specimens. Cancer had relapsed in three patients, as indicated by rising serum total prostate specific antigen (PSA) levels in less than five years even though the post-RP pathology report did not detect cancer cell invasion to prostate margin/capsule, seminal vesicle, and/or pelvic lymph node. Ratios of CB to SA were significantly higher in cancer than in BPH. Study 2. In the Gleason score 6 and 7 tumors, the ratios of CB to SA were not significantly different in biopsies from those in prostectomies, indicating the reliability of immunostaining in small tissue sections.
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 01, 2006
- Accession Number
- ADA454847
Entities
People
- Akhouri A. Sinha
Organizations
- University of Minnesota