CTL - Tumor Cell Interaction: The Generation of Molecular Probes Capable of Monitoring the HLA-A*0201-HER-2/neu Peptide Complex
Abstract
The overall goal of this project is to probe the CTL - tumor cell interaction by generating scFv probes that are able to recognize the HLA- A*0201-HER-2/neu369-377 peptide complex. In the 12 month period covered by this report I aimed to improve the affinity of HLA-A*0201- HER-2/neu369-377 peptide complex-specific scFv 2.3.5 which was isolated during the first 12 month period of this project. Utilizing three rounds of site-directed mutagenesis in the VL CDR3 and VL CDR1 of scFv 2.3.5, I have generated and isolated a scFv clone named 2.3.5- 58-53 which exhibited markedly higher affinity for the HLA-A*0201-HER-2/neu369-377 peptide complex than the parental scFv 2.3.5. I will utilize scFv 2.3.5-58-53 to analyze the levels of HLA-A*0201 :HER-2/neu369-377 complexes expressed by breast carcinoma cell lines and breast carcinoma lesions in order to determine if there are correlations between the levels of antigen processing machinery components HLA class I antigens Beta2-microglobulin and/or HER-2/neu on the levels of HLA-A*0201 :HER-2/neu369-377 complex on breast carcinoma cells.
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 01, 2006
- Accession Number
- ADA454867
Entities
People
- Eric C. Ko
Organizations
- Roswell Park Comprehensive Cancer Center