Breast Cancer Therapy Using Antibody-Endostatin Fusion Proteins
Abstract
To produce a more effective form of Herceptin and improve efficacy of human endostatin, we have constructed an anti-HER2 IgG3-human endostatin fusion protein by joining human endostatin to the 3 end of humanized anti-HER2 IgG3. The wild type and the mutant type (P125A) of human endostatin were constructed with FLAG tagging to distinguish from the endogenous human endostatin. The anti-HER2 IgG3-huEndo fusion proteins were constructed as wild type and mutant type (P125A) and were stably transfected into B myeloma cells. The secreted huEndo fusion proteins have a molecular weight of 220 kDa for the anti-HER2 IgG3-CH3-huEndo fusion protein and 160 kDa for the anti-HER2 IgG3-Hinge-huEndo fusion protein. The huEndo fusion proteins were faithfully secreted as the fully assembled H2L2 form.
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 01, 2006
- Accession Number
- ADA455083
Entities
People
- Seung-uon Shin
Organizations
- University of Miami