Genetically Targeted Radiotherapy Utilizing the Human Sodium Iodide Symporter in Human Breast Carcinoma Cells
Abstract
The purpose of this proposal was to examine the efficiency of NIS mediated genetically targeted radiotherapy as a possible non-invasive therapeutic treatment in human breast carcinoma. SK-Br-3 cells were transfected with hNlS plasmid to develop stable NIS expressing cells. Clones were grown and are currently being screen for radioactive accumulation. The stable NIS-expressing cells will then be utilized in determining the level of NIS expression necessary to elicit a bystander effect. In addition to creating stable NIS-expressing cells treatments with various histone deacetylase inhibitors or retinoic acid were used to increase the endogenous NIS expression in the breast carcinomas cells. Real time RT-PCR was utilized to analysis the NIS mRNA expression following the treatments. Radioiodide accumulation assays were then performed to examine the ability of the cells to uptake radioactivity. Ad-NIS treatment coupled with increases in endogenous NIS may help to improve the therapeutic outcome.
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 01, 2006
- Accession Number
- ADA455269
Entities
People
- Frederick E. Domann
- Kimberly Krager
Organizations
- University of Iowa