Molecular Aspects of Muscle Damage and Denervation with Public Access Tools
Abstract
The over-riding hypothesis of this proposal is that muscle tissue, and its major cell type (the myofiber) is an ideal platform on which to test the power of post-genomic research, including integration of DNA, mRNA, and protein data. This tissue is also of considerable importance to the military (muscle function of personnel) and to their families (muscular dystrophy is among the most common inherited disorders). An important aspect of post-genomic research is the rapid release of large amounts of data into the public sector, usually via integrated web-accessible databases, typically prior to publication. We have made the first major steps in public access to highly dimensional mRNA data through the construction and implementation of a QC/SOP standardized platform mRNA profile public Oracle web database, currently populated by 887 Affymetrix profiles from human, mouse, and rat. In this current proposal, we request funds for two specific aims. The first is to build upon our experience with our integrated lab mRNA profile Oracle LIMS and Oracle web database, and re-design the structure of the database to enable higher order data analysis tools. This first aim is relevant to the military, as it will provide a national resource for understanding of muscle and nerve function and dysfunction in use, disuse, trauma, regeneration, and dystrophy. The second aim is to extend the integrate data resource from mRNA to protein, using a high throughput ABI TOF/TOF mass spec with comparative proteomics with O18 labeled tryptic digests of protein fractions from normal, atrophic, and dystrophic muscle. This latter aim is also relevant to the military due to the increased understanding of muscle remodeling following specific stimuli that are frequently encountered in the field (loss of weight-bearing following trauma, and plasticity in response to damage).
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 01, 2004
- Accession Number
- ADA455552
Entities
People
- Eric Hoffman