Genome-Wide Chromosomal Targets of Oncogenic Transcription Factors
Abstract
We proposed to develop a new genomic method named STAGE (Sequence Tag Analysis of Genomic Enrichment) to identify the direct downstream targets of transcription factors that are important in breast cancer. STAGE is based on high-throughput sequencing of concatamerized tags derived from DNA associated with transcription factors that is isolated by chromatin immunoprecipitation. Identification of the direct targets of oncogenic transcription factors important in breast cancer can help elucidate their role in mediating gene regulatory networks underlying the disease. Our work over the past year has successfully identified targets of c-Myc, E2F4 and Stat1, all factors important in breast cancer, and our data supports the idea that it is possible to comprehensively identify the targets of transcription factors important in breast cancer using STAGE, as proposed in our Statement of Work.
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 01, 2006
- Accession Number
- ADA455791
Entities
People
- Vishwanath R. Lyer
Organizations
- University of Texas at Austin