Identifying Molecular Targets for Chemoprevention in a Rat Model

Abstract

The purpose of this grant is to determine the molecular events that occur in the dorsal and ventral lobes of the rat prostate gland after 20 weeks of exposure to PhIP (2-amino-i -methyl-6-phenylimidazo[4,5-b]pyndene). PhIP is a potent inducer of mutations in the rat prostate where we have shown that it forms bulky DNA adducts. In addition it induces high levels of oxidative damage in the target tissues. The scope of this research includes: 1) Generation of a rat model 2) Analysis of the rats prostate after 20 weeks of PhIP and 3) Gene chip microarray analysis. To date we have completed the histopathologic analysis of PhIP induced prostate pathology we have generated an inflammatory/atrophy/proliferation model and we have acquired data that refutes a previous model of neoplastic progression. These results have been summarized in a paper submitted to the journal Neoplasia.

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Document Details

Document Type
Technical Report
Publication Date
Dec 01, 2005
Accession Number
ADA456857

Entities

People

  • Ralph W. Devere White

Organizations

  • University of California

Tags

DTIC Thesaurus Topics

  • Body Weight
  • Carcinoma
  • Cells
  • Department Of Defense
  • Diseases And Disorders
  • Dna Microarrays
  • Epithelial Cells
  • Glands
  • Histology
  • Laboratory Animals
  • Microarray Analysis
  • Mutations
  • Neoplasms
  • Prostate
  • Prostate Cancer
  • Prostate Gland
  • Tissues

Fields of Study

  • Biology
  • Medicine

Readers

  • Computational Modeling and Simulation
  • Prostate Cancer Biology.
  • Toxicology/Environmental Toxicology

Technology Areas

  • Biotechnology