Loss of Nucleotide Excision Repair as a Source of Genomic Instability in Breast Cancer
Abstract
Altered nucleotide excision repair (N ER) activity may be a common, initial and detectable event leading to genomic instability in human breast epithelial cells, ductal carcinoma in situ or early invasive breast cancers. Our objective is to study DNA repair activity in primary breast epithelial cells and cancer tissues from women at risk for or diagnosed with breast cancer to determine if NER activity can be reliably and practically quantitated from these tissues and cells. The three specific aims are: I) Apply a quantitative local DNA damage immunoflourescent assay to measure NER activity in single cells derived from women at risk for or with breast cancer, from ductal lavage or MR directed biopsy samples of breast epithelial cells or tumor cells, and from appropriate controls. 2) Develop a flow cytometry based immunoassay to measure NER activity in small populations of cells derived from women at risk for or with breast cancer, from ductal lavage or MR directed biopsy samples of breast epithelial cells or tumor cells, and from appropriate controls. 3) Analyze cell based NER activity from patients samples with clinical, pathological and genetic information, including BROAl and BRCA2 carrier status, pathologic grade and stage, familial risk, and molecular correlates
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 01, 2006
- Accession Number
- ADA456858
Entities
People
- James M. Ford
Organizations
- Stanford University