The Role of the Low Molecular Weight (LMW) Isoforms of Cyclin E in Breast Cancer Tumorigenesis
Abstract
Cyclin E is a positive regulator of the G1 to S phase transition of the cell cycle. In complex with CDK2 it is responsible for cells passing the restriction point, committing the cell to a round of DNA replication. Previously this laboratory found that cyclin E is overexpressed and present in lower molecular weight (LMW) isoforms in breast cancer cells and tumor tissues compared to normal cells and tissues. To investigate the role of the LMW forms of cyclin E in tumorigenesis we have developed a model system of non-tumorigenic breast cells overexpressing the individual isoforms of cyclin E. Using this model system we have determined that the LMW forms of cyclin E cause increased proliferation of non-tumorigenic breast epithelial cells. This proliferation can be inhibited by preventing the processing of the LMW forms of cyclin E through the expression of elafin in these cells.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 01, 2006
- Accession Number
- ADA457665
Entities
People
- Hannah Wingate
Organizations
- The University of Texas MD Anderson Cancer Center