DARPA BioComp Plasmid Distribution 1.00 of Standard Biobrick Components
Abstract
This distribution consists of 300 ng aliquots of plasmid DNA for each of twelve components and compound constructs utilizing our idempotent assembly strategy. The dried DNA should be stable at room temperature for many weeks, but long term storage at -20 or -80 in the resealable foil pouch with desiccant is recommended. Much of the power of these assembly techniques arise from a consistent, widely available set of components. We strongly encourage others who develop components in this form to contact us with complete information and samples, if possible. We volunteer to act as a community coordination and distribution point for these components as they are developed. If you have components which you believe are useful but not in this form, we may, in many cases, be interested in doing the work necessary to cast them into standard component form. We are working to develop licenses which would encourage widespread free distribution of DNA components, sequences, and documentation and intend those licenses, as developed, to apply to these components and assembly strategies.
Document Details
- Document Type
- Technical Report
- Publication Date
- May 22, 2002
- Accession Number
- ADA457854
Entities
People
- Tom Knight
Organizations
- Massachusetts Institute of Technology