Bone Sialoproteins and Breast Cancer Detection
Abstract
We have been studying a family of proteins that we have termed SIBLINGs for Small Integrin Binding Llgand N-linked Glycoproteins that share similar structural domains human chromosomal location normal synthesis by skeletal tissue and abnormal expression by neoplasms . The goal of our research is to test whether SIBLINGs might be informative markers for breast cancer detection. To accomplish this goal we have developed competitive enzyme-linked immunosorbent assays (ELISAs) for the SIBLINGs bone sialoprotein (BSP) osteopontin (OPN) dentin matrix protein-1 (DMP1) dentin sialophosphoprotein (DSPP) and matrix extracellular phosphoglycoprotein (MEPE). Sandwich-based ELISA assays have also been developed. When the competitive ELISAs were used to screen SIBLING protein levels BSP and OPN exhibited the highest degree of sensitivity and specificity for the detection of breast cancer. Microarray analysis of normal and breast cancer-derived mRNA samples found a similar elevated levels of elevated SIBLING expression. The levels of certain SIBLINGs in serum were found to be correlated with cancer stage. These results suggest that SIBLINGs may have utility as serum-based markers for breast cancer detection.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 2006
- Accession Number
- ADA458172
Entities
People
- Neal S. Fedarko
Organizations
- Johns Hopkins University