Suppression of Breast Cancer Progression by Tissue Factor
Abstract
Tissue Factor (TF) is the cell surface receptor that activates coagulation by binding the serine protease coagulation factor Vila (Vila). The activation of the coagulation cascade leads to thrombin generation, fibrin formation and platelet activation which together aide tumor growth and metastasis.While the role of TF in metastasis through thrombin pathways is well established, evidence is increasing that TF may drive tumor development dependent on cell signaling pathways. A newly developed breast cancer model with a tetracycline regulated TF expression cassette shows TF enhances breast cancer tumor growth. This model will be useful to mechanisms by which TF enhances breast cancer progresssion. In this grant, we further evaluated the role of the TF cytoplasmic domain in breast cancer progresssion. We established tumor prone transgenic models in the C57B1/6 background and compared tumor development in TF cytoplasmic domain deleted mice with wild-type animals. Consistent with a recent report, we found that the C57B116 03-TAg model is unsuitable for studying breast cancer, because mice developed debilitating chondromatosis prior to the appearance of breast tumors. Experiments are ongoing to evaluate the role of the TF cytoplasmic domain in breast cancer development and progression to metastatic disease in the PyMT model.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 01, 2006
- Accession Number
- ADA458396
Entities
People
- Wolfram Ruf
Organizations
- Scripps Research