The Role of the POZ-ZF Transcription Factor Kaiso in Breast Cell Proliferation and Tumorigenesis

Abstract

Kaiso is a new member of the POZ-ZF transcription factor family, which was first identified as a binding partner for the cell adhesion co-factor p120ctn. Preliminary work from our lab indicated that Kaiso is misexpressed in ~40% of human breast tumors and identified cyclin D1 as a putative Kaiso target gene. The purpose of this project is to elucidate the mechanisms by which Kaiso's transcriptional regulation of cyclin D1 affects breast cell proliferation and contributes to breast tumorigenesis. Using such techniques as artificial promoter assays and semi-quantitative RT-PCR, we sought to determine how Kaiso regulates cyclin D1 promoter binding and expression. Our work to date demonstrates that Kaiso is a transcriptional repressor of the cyclin D1 gene and implicates Kaiso and p120ctn activity in modulating canonical Wnt signaling.

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Document Details

Document Type
Technical Report
Publication Date
Mar 01, 2006
Accession Number
ADA458467

Entities

People

  • Abena Otchere

Organizations

  • McMaster University

Tags

DTIC Thesaurus Topics

  • Adhesion
  • Biomedical Research
  • Breast Cancer
  • Cell Line
  • Cell Physiological Processes
  • Cells
  • Epithelial Cells
  • Medical Personnel
  • Neoplasms
  • Proteins
  • Regulations
  • Transcription Factors
  • Tumor Cell Line

Fields of Study

  • Biology

Readers

  • Molecular Biology and Genetics