EGFR Activation by Spatially Restricted Ligands

Abstract

Misregulation of Transforming Growth Factor alpha (TGFalpha) and increased Epidermal Growth Factor Receptor (Egfr) activity has been associated with an increased prognosis of breast cancer. During cogenesis in Drosophila melanogaster local Egfr activation by the spatially-restricted TGFalpha-like ligand Gurken (Grk) is required for axis formation in the egg and future embryo. Squid (Sqd) a heterogeneous nuclear ribonucleoprotein (hnRNF) functions in the localization and translational regulation of grk mRNA. The purpose of this project is to identify factors that function with Sqd to produce spatially-restricted Egfr activation. We have integrated genetic and biochemical methods to identify and characterize the following factors that interact with Sqd: (1) an arginine methyltransferase CG6554 which may serve to methylate Sqd in vivo, (2) Hrb27C an hnRNF protein and Ovarian tumor which are required for proper grk expression and (3) Bruno Cup and polyA-binding protein which function together to mediate grk translational regulation.

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Document Details

Document Type
Technical Report
Publication Date
Jun 01, 2006
Accession Number
ADA459169

Entities

People

  • Jennifer S. Goodrich
  • Katherine N. Clouse

Organizations

  • Princeton University

Tags

DTIC Thesaurus Topics

  • Breast Cancer
  • Carrier Proteins
  • Cell Nucleus
  • Cells
  • Chemistry
  • Chromosome Structures
  • Chromosomes
  • Diptera
  • Genetic Structures
  • Genetics
  • Growth Factors
  • Health Services
  • Neoplasms
  • Peptide Growth Factors
  • Peptides
  • Proteins
  • Rodents

Fields of Study

  • Biology

Readers

  • Breast cancer cell signaling and growth regulation.

Technology Areas

  • Biotechnology