Process Research and Development of Antibodies as Countermeasures for C. Botulinum

Abstract

This report describes the design of a CGMP facility for yeast and bacteria on the first floor and basement of Othmer Hall, the home for the University of Nebraska-Lincoln Biological Process Development Facility. Design and a preliminary cost estimate is completed and the Program statement for the project is being submitted to the University of Nebraska Board of Reagents for approval. This report also describes research on the expression of antibodies in Chinese Hamster Ovary (OH 0) cells against serotype A botulinum neurotoxin. We have completed construction of a simplified and easy to use CHO expression plasmid for the light and heavy chain, a system for selection of higher expression clones using dfr and zeocin for selection and GFP (green fluorescence protein) and flow cytometery for selection of higher expressing clones, and finally a development of a defined media for fed-batch production of mAb in CHO.

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Document Details

Document Type
Technical Report
Publication Date
Mar 01, 2006
Accession Number
ADA459188

Entities

People

  • Michael Meagher

Organizations

  • University of Nebraska–Lincoln

Tags

DTIC Thesaurus Topics

  • Amino Acids
  • Bacteria
  • Cell Membrane
  • Chemical Synthesis
  • Chemistry
  • Culture Media
  • Culture Techniques
  • Essential Amino Acids
  • Fatty Acids
  • Fungi
  • Health Services
  • Proteins

Fields of Study

  • Biology

Readers

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  • Nuclear Civil Defense.
  • Software Engineering