Molecular Mechanism by Which Retinoids Prevent Breast Cancer Development
Abstract
My goal of the study is to know whether LGD 1069 and LG100268 (rexinoids) suppress the growth of breast cancer cells, and how these drugs act to inhibit breast cancer development. For the purpose, I tested 5 different breast cell lines: 1 human mammary epithelial cell line(HMEC), 2 different ER-positive cell lines (MCF-7 and T47D) and 2 different ER- negative breast cancer cell lines (MDA-MB-231 and MDA-MB-435). By MTS assay, I found that both LGD1069 and LG100268 inhibited significantly normal HMEC cell growth 10 uM. I also found that LGD1069 strongly suppressed the growth of T47D (Er-positive) by dose dependent manner. LGD1069 also induced a mild inhibition of MDA-MB-231(ER-negative) at 10 uM MCF-7 and MDA-MB-435 did not have growth suppression by LGD1069 at 10 uM. LG100268 did affect little the cell growth in all 4 breast cancer cell lines suggesting its weak activity compare to LGD1069. To investigate the mechanism by which LGD1069 suppresses breast tumorigenesis, we have studied the genes modulated by LGD1069. We treated 2 different cell lines (t47D and MDA-MB-231) with LGD1069 at 10 uM for 12 hrs, extracted RNA and then submitted to Affymetrix Microarray. According to the data, we found several interesting genes induced by LGD1069. We will study their functions. In this report, I present genes regulated by LGD1069 in MDA-MB-231, ER-negative cell line.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 01, 2006
- Accession Number
- ADA459784
Entities
People
- Hye-sook Seo
Organizations
- The University of Texas MD Anderson Cancer Center