Dysregulation of RNA Interference in Breast Cancer

Abstract

The newly discovered RNA interference is a novel type of gene regulation mechanism, which is required for normal expression of genes. This study tests the hypothesis that breast tumor carries dysregulated RNA interference pathways, and thus, some tumor suppressor genes will be down-regulated while other genes (e.g., oncogenes) will be up-regulated, leading to tumor cell proliferation and survival. Using real time RT-PCR, we demonstrate that microRNA-21 is overexpressed in breast tumors compared to the matched normal breast tissue. Furthermore, we show that antisense oligonucleotide against microRNA-21 can suppress the endogenous microRNA-21 and causes tumor cell growth inhibition. Experiments with a xenograft carcinoma mouse model reveal that the antisense microRNA-21 oligonucleotide also inhibits tumor growth. Therefore, microRNA-21 is a potential therapeutic target for breast cancer therapy.

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Document Details

Document Type
Technical Report
Publication Date
Jul 01, 2006
Accession Number
ADA460075

Entities

People

  • Yin-Yuan Mo

Organizations

  • Southern Illinois University

Tags

DTIC Thesaurus Topics

  • Antineoplastic Agents
  • Apoptosis
  • Biomedical Research
  • Breast Cancer
  • Cancer
  • Cell Line
  • Cell Physiological Processes
  • Cells
  • Gene Expression
  • Inhibition
  • Inhibitors
  • Neoplasms
  • Nucleic Acids
  • Regulations
  • Stem Cells
  • Tumor Cell Line
  • Xenografts

Fields of Study

  • Biology

Readers

  • Molecular Biology and Genetics