Chromatin Regulation of EGFR Locus in Human Mammary Epithelial Cells
Abstract
Relationship between chromatin remodeling and mammary tissue-specific gene transcription is not well understood. Using milk protein beta-casein as a marker, we investigate how extracellular matrix (ECM) and lactogenic hormone control transcription factors activity, and elucidate the role of histone acetylation and A P-dependent chromatin remodeling in the transcriptional regulation. By ChIP assays, we show that ECM cooperates with prolactin to induce binding of Stat5 and C/EBPBeta in the Beta-casein promoter. We also show that the levels of acetylated histones increase in the Beta-casein promoter. However, increasing acetylated histone levels in the promoter region by TSA treatment failed to induce Beta-casein expression, suggesting histone acetylation is not sufficient for the gene transcription. Introduction of the ATPase-deficient SWI/SNF complex significantly blocked Beta-casein expression, indicating that ATP-dependent chromatin remodeling is required for the transcriptional activation of this gene. Taken together, these observations indicate that Beta-casein expression requires the concerted action of both transcription and chromatin remodeling factors.
Document Details
- Document Type
- Technical Report
- Publication Date
- May 01, 2005
- Accession Number
- ADA460782
Entities
People
- Mina Bissell
- Ran Xu
Organizations
- University of California, Berkeley