Identifying Early Diagnosis Markers of Prostate Cancer

Abstract

The successful treatment of prostate cancer requires detection of the disease at early stages. Currently the early diagnosis of prostate cancer largely depends on the detection of prostate-specific antigen (PSA) in circulation. However, PSA can only precisely detect 40% of prostate cancer and is not specific for the occurrence of prostate cancer. We reasoned that the success and accuracy in early diagnosis of prostate cancer may be significantly improved if a panel of prostate cancer-specific markers can be identified and used in combination for detecting early stage of prostate cancer. In the first year of the funding period, we constructed cDNA library in our pTRAP1 retroviral plasmid using RNA isolated from human prostate tumor samples. In the second year, we generated human prostate tumor cDNA library in which the signal peptides are enriched. In the third year of this funding, we screened our generated prostrate tumor library and identified 10 either secreted or cell surface proteins overexpressed in prostrate tumors. Currently, we are in the process to validate our findings and hope using these proteins as early diagnosis biomarkers for prostate cancer.

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Document Details

Document Type
Technical Report
Publication Date
Jul 01, 2006
Accession Number
ADA462063

Entities

People

  • Shuang Huang

Organizations

  • Scripps Research

Tags

DTIC Thesaurus Topics

  • Accuracy
  • Biological Factors
  • Biological Markers
  • Biomedical Research
  • Cells
  • Detection
  • Diseases And Disorders
  • Epithelial Cells
  • Gel Electrophoresis
  • Genetic Structures
  • Growth Factors
  • Medical Personnel
  • Neoplasms
  • Prostate
  • Prostate Cancer
  • Statistical Analysis
  • Tissues

Fields of Study

  • Biology
  • Medicine

Readers

  • Molecular Genetics
  • Oncology and Biomarker-Based Cancer Detection.