Bacterial Degradation of DNT and TNT Mixtures

Abstract

The major objective of this research project was to develop bacterial strains with the ability to efficiently degrade mixtures of dinitrotoluene (DNT) isomers and expand that degradation capability to include TNT. Since most contaminated sites contain mixtures of nitroarene compounds, these strains would have the potential for use in the bioremediation of field sites. We have isolated strains that can degrade several nitroarene compounds, including nitrobenzene, 2-nitrotoluene (2NT) and 2,4- and 2,6-DNT. A clear understanding of the biochemistry, physiology, and molecular genetics of these pathways is a necessary prerequisite for pathway engineering. To this end, we characterized the pathways and enzymes involved, investigated regulation systems, and identified the inducing molecules.

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Document Details

Document Type
Technical Report
Publication Date
Oct 31, 2005
Accession Number
ADA462201

Entities

People

  • Glenn R. Johnson
  • Jim C. Spain
  • Rebecca E. Parales

Organizations

  • University of California, Davis

Tags

Communities of Interest

  • Energy and Power Technologies

DTIC Thesaurus Topics

  • Anti-Bacterial Agents
  • Aromatic Compounds
  • Aromatic Hydrocarbons
  • Bacteria
  • Biodegradation
  • Chemical Analysis
  • Chemical Synthesis
  • Chemistry
  • Dna Sequence Analysis
  • Genetic Engineering
  • Genetics
  • Liquid Chromatography
  • Mass Spectrometry
  • Metabolism
  • Microbiology
  • Pseudomonas Infections

Fields of Study

  • Environmental science

Readers

  • Agricultural Chemistry/Soil Science
  • Microbial Pathology
  • Organic Chemistry

Technology Areas

  • Biotechnology
  • Biotechnology - Bioremediation