Proteinated Subnano Particles of Elemental Selenium for the Treatment of Breast Cancer

Abstract

The purpose of this award is to test in preclinical models the hypothesis that cytotoxic conjugates of elemental selenium and proteins are safe and effective for the systemic therapy of invasive breast cancer. The grant has three specific aims, 1) to evaluate the safety and efficacy of systemically administered Se(0)- protein conjugates in athymic nude mice bearing xenografts of human breast cancer cells, 2) to assess the functional integrity of conjugate-treated normal human hematopoietic stem cells, and 3) to determine by use of the combination index method how Se(0)-protein conjugates interact with standard chemotherapeutic agents that are commonly used in the treatment of invasive breast cancer. We report here on the preparation and evaluation of high potency cytotoxic Se(0)-protein conjugates, a quantitative in vitro analysis of the interactions of Se(0)-protein conjugates with other chemotherapeutic agents, the surprise finding that certain antibiotics can interfere with the generation of cytotoxic conjugates, and a simple and rapid spectroscopic method to detect such interferences.

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Document Details

Document Type
Technical Report
Publication Date
Sep 01, 2006
Accession Number
ADA462390

Entities

People

  • Fritz Sieber

Organizations

  • Medical College of Wisconsin

Tags

DTIC Thesaurus Topics

  • Albumins
  • Anti-Bacterial Agents
  • Breast Cancer
  • Cell Line
  • Cells
  • Chemotherapeutic Agents
  • Chemotherapy
  • Culture Techniques
  • Diseases And Disorders
  • Infection
  • Neoplasms
  • Proteins
  • Selenium
  • Standards
  • Stem Cells
  • Test And Evaluation
  • Transplants

Readers

  • Oncology (Cancer Research).

Technology Areas

  • Biotechnology
  • Biotechnology - Cancer Biotech