Prognostic Value of the K303R Estrogen Receptor Alpha Mutation in Breast Cancer
Abstract
The estrogen receptor (ER)-alpha plays a major role in breast tumor progression, we have recently discovered a somatic mutation (A908G) that leads to a lysine to arginine (K303R) amino acid change. Here we proposed to study if the K303R ER-alpha mutation is prognostic clinical factor for invasive breast cancer. We have determined that the mutation is present in approximately 56% of invasive breast cancers and its expression correlates with lymph node involvement. Expression constructs containing the wild-type or mutant ER-alpha fused to a yellow-fluorescent protein have been developed and stably transfected into MCF-7 breast cancer cells. In vivo nude mouse studies are underway to analyze experimental metastases. Specific Aim 1 has previously been completed and the mutation frequency has been determined in Aim 2. Additional analysis of clinical parameters is ongoing. Specific Aim 3 is underway and mice will be analyzed for experimental metastases in the near future.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 01, 2005
- Accession Number
- ADA462399
Entities
People
- Matthew H. Herynk
- Suzanne A. Fuqua
Organizations
- Baylor College of Medicine