Nuclear Dynamics of BRCA1-Dependent Transcription Regulation
Abstract
BRCA1 coordinates cellular responses to DNA damage. It functions as a co-repressor of GADD45atranscription through interactions with a DNA-binding protein termed ZBRK1. Our goal is to develop a biosensor system to visualize transcription control by ZBRK1 and BRCA1 in single living and/or fixed cells. The rationale is to use integrated DNA binding sites to obtain real time, multiplex-based data. The reportable outcomes for the period are: 1) UAS and ZREarray-bearing plasmids have been constructed; 2) Transient and stable reporter expression have been demonstrated; 3) Stable cell lines with G20, G40 and Z32 are on line for studies;4) Construction of fluorescent GAL4-DBD and ZBRK1 fusion protein has been achieved, and BRCA1 derivatives are in progress. When operational, this system will document real time nuclear dynamics of ZBRK1/BRCA1-dependent chromatin modification systems, as cells mount transcriptional responses to genotoxins.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 2005
- Accession Number
- ADA462433
Entities
People
- Zelton D. Sharp
Organizations
- University of Texas Health Science Center at San Antonio