Tissue Factor Plasma Analysis
Abstract
This progress report describes the assays, protocols and results related to a collaborative research initiative between Dr. Mann s research laboratories (coagulation research laboratory and Antibody Core Facility, Department of Biochemistry, University of Vermont, Burlington, Vermont) and Dr. Park s clinical research involving trauma and acutely burned patients (U. S. Army Institute of Surgical research, Fort Sam Houston, Texas). The major areas of research involved assessment of the role of soluble and membrane-bound tissue factor (TF) in the overall responses of trauma and burned patients to interventions and to determine whether TF concentrations in blood and/or on cell over time can predict the risks for developing Multi-Organ Dysfunction syndrome (MODS) or Adult Respiratory Distress Syndrome (ARDS). Dr. Mann s laboratory has determined the TF antigen concentrations in plasmas from 149 trauma and burned patients. Each patient had several plasma samples that had been collected at various times following hospitalization. These samples include baseline (the day of hospitalization), day 1, day 3, day 5, day 7, and plasma samples drawn in between hospitalization days and the discharge days (in most cases up to 42 days). The total numbers of plasmas tested were 514. For each sample duplicate determinations were performed. The TF activity was also determined for 514 plasmas and when TF activity was detected, anti-TF monoclonal antibody (mAb) was used to verify the observation. If active TF was found in plasma, anti-TF mAb should inhibit the activity. These data were provided to Dr. Park for further analyses of the correlation of the TF antigen and activity with clinical data obtained by Dr. Park s team.
Document Details
- Document Type
- Technical Report
- Publication Date
- Nov 01, 2006
- Accession Number
- ADA462446
Entities
People
- Kenneth G. Mann
- Sarah A. Cooley
Organizations
- University of Vermont