SRD5A1 Genetic Variation and Prostate Cancer Epidemiology
Abstract
The human steroid 5-alpha reductase type I (SRD5A1) gene was sequenced in 101 men in order to identify genetic variants that might predispose carriers to prostate cancer. In these analyses, we uncovered a series of single nucleotide polymorphisms (SNPs) in the 3 -untranslated region (3 -UTR) of the SRD5A1 mRNA. However, we did not find SNPs that changed amino acid identities of the SRD5A1 protein. To further pursue the relevance of the SNPs in the 3 -UTR of this gene, we used cell culture assays to measure how they may alter RNA half-life, steady-state, and translation. Different combinations of 3 -UTR SNPs ( RNA haplotypes ) resulted in reduced SRD5A1 mRNA half-life and steady state levels in our assay system. This data is consistent with the hypothesis that SNPs in the 3 -UTRs of mRNAs may play a role in the regulation of the SRD5A1 gene. Therefore, these SNPs should be considered as candidates for having biological function that might predispose to prostate cancer.
Document Details
- Document Type
- Technical Report
- Publication Date
- May 01, 2006
- Accession Number
- ADA462447
Entities
People
- Troy Phipps
Organizations
- University of Southern California