Protein Adducts of the Prostate Carcinogen PhlP in Children
Abstract
The goal of this project is to develop an assay to measure the binding a common food-borne carcinogen (PhIP)to human serum albumin (HSA) an abundant protein in blood that frequently binds to small molecules in the blood stream. Measurement of HSA-bound PhIP may allow determination of the exposure (dosimetry) of large populations of humans to PhIP. Such critical data are almost never included in epidemiology studies owing to low detection sensitivity of current methods. Based upon our earlier results in humans demonstrating that PhIP binds to HSA we are developing an ultrasensitive mass spectrometry assay that depends upon competitive binding of adducted HSA to an antibody saturated with a radiocarbon labeled PhIP-bound peptide. This assay will allow quantitative measurement with the naturally occurring substrate from archived human samples. We report progress towards antibody development that will enable the assay to be realized.
Document Details
- Document Type
- Technical Report
- Publication Date
- Feb 01, 2006
- Accession Number
- ADA462546
Entities
People
- Paul T. Henderson
Organizations
- University of California