Identification and Characterization of Ovarian Carcinoma Peptide Epitopes Recognized by Cytotoxic T Lymphocytes
Abstract
The purpose of the research is to identify new ovarian cancer tumor antigens that can be used in the immunotherapeutic treatment of ovarian cancer. The scope of this work involves (1) identifying the peptide antigens recognized by ovarian reactive cytotoxic T lymphocytes (CTL) by using an antigen-unbiased mass spectrometric approach to antigen identification; and (2) identify peptide antigens within the Her-2/neu folate binding protein (FBP) and TAG proteins that give rise to ovarian reactive CTL. A total of twelve ovarian cancer lines have been characterized for their expression of class I MHC molecules and tumor antigens. Three TAG-derived epitopes including two HLA-A2-restricted epitopes (SLGWLFLLL and LLLRLECNV) and one HLA-B7-restricted epitope (LPAQEGAPT) were identified. T cell lines are being established from the TAL of ovarian cancer patients. In preliminary experiments two of these lines appear to recognize the FBP-derived peptide FLLSLALML and the mesothelin-derived peptide FLLFSLGWV. The significance of these results are that they provide new antigens for use in therapeutic vaccine trials for the treatment of ovarian cancer. Furthermore they provide the reagents necessary to assess the immunologic impact of such trials.
Document Details
- Document Type
- Technical Report
- Publication Date
- Nov 01, 2006
- Accession Number
- ADA462674
Entities
People
- Kevin T. Hogan
Organizations
- University of Virginia