Amplification of Type II Cadherins in Prostate Cancer
Abstract
Genomic alterations of chromosome 18q have been observed in prostate cancer. This research focuses on analyzing the role of increased gene copy number at chromosome 18q22.1 in prostate cancer. We believe the key genes in this region are the type II cadherins. We are studying the role of overexpression of these genes, particularly cadherin 7 (CDH7), on the tumorigenicand invasive potential of prostate cancer cells. We have shown the minimal region of increased copy number contains CDH7. This increased copy number of CDH7 is specific to prostate cancer and is not found in II other common cancers. The increased copy number of CDH7 also results in increased levels of cadherin-7 mRNA in prostate tumors. We are purifying polyclonal antibodies against cadherin-7 to use in immunohistochemistry experiments to determine if increased CDH7 copies results in increased levels of protein. We performed knockdown experiments of the cadherin-7 mRNA in a prostate cancer cell line and are analyzingthe cadherin-7 protein levels in these cells. We will subsequently evaluate these cellswith reduced cadherin-7 expression for their invasive and tumorigenic potential.
Document Details
- Document Type
- Technical Report
- Publication Date
- Nov 01, 2006
- Accession Number
- ADA462675
Entities
People
- Teresa L Johnson-Pais
Organizations
- University of Texas Health Science Center at San Antonio