Production of a DNA Vaccine Specific for the 64 kDa Protective Antigen of Erysipelothrix rhusiopathiae

Abstract

The gene for the protective antigen of E. rhusiopathiae will be inserted into a eukaryotic vector both for the production of a DNA vaccine and for large scale production of the recombinant protein (in vitro). The DNA vaccine will be tested in cetacean cell lines for its ability to transfect the cells. The recombinant protein will be used to improve the existing ELISA for determining the antibody titers in cetacean serum samples to E. rhusiopathiae.

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Document Details

Document Type
Technical Report
Publication Date
Feb 08, 2007
Accession Number
ADA462832

Entities

People

  • Bobby L. Middlebrooks

Organizations

  • University of Southern Mississippi

Tags

DTIC Thesaurus Topics

  • Abstracts
  • Animals
  • Cell Line
  • Cells
  • Cetaceans
  • Column Chromatography
  • Genetic Structures
  • Mammals
  • Marine Mammals
  • Military Research
  • Mississippi
  • Polymerase Chain Reaction
  • Production
  • Proteins
  • Recombinant Proteins
  • Universities
  • Vaccines

Fields of Study

  • Biology
  • Engineering

Readers

  • Immunology
  • Molecular Genetics

Technology Areas

  • Biotechnology