Diversity, Replication, Pathogenicity and Cell Biology of Crimean Congo Hemorrhagic Fever Virus
Abstract
This research project is a result of a collaboration between three research groups aimed at elucidating basic replication processes of CCHFV with the expected outcome of providing basic research reagents and establishing the foundation of knowledge necessary for discovery of vaccines and antiviral therapeutics for Crimean Congo hemorrhagic fever. Our major findings during the second year of support are the following: We have mapped domains in the N and L proteins of CCHFV responsible for protein-protein interactions and RNA-protein interactions. We have identified a novel activity associated with the N-terminal of the L protein, that is responsible for deconjugation of ubiquitin and ISG15 conjugates, that could be a target for antiviral development and for attenuation. We have detected and NSm protein produced after cleavage of the glycoprotein precursor in virus infected cells. The NSm is stable and transported to the Golgi. We have optimized the techniques to propagate CCHFV in tissue culture and generated high titer stocks. Our results provide novel insights on the molecular biology of this understudied highly pathogenic human virus.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 2006
- Accession Number
- ADA462887
Entities
People
- Adolfo García-Sastre
Organizations
- Icahn School of Medicine at Mount Sinai