Microlocalization and Quantitation of Risk Associated Elements in Gleason Graded Prostate Tissue
Abstract
The objective of this proposal was to determine the concentration and location of elements in normal and tumor tissue. Specific aims included: (1) preparation of Gleason graded prostate tissue, (2) determination of tissue concentrations of: B, Ca, Cd, Se and Zn; and (3) determination of tissue and cellular distribution of these elements using a NanoSIMS ion microscope at Lawrence Livermore National Laboratory (LLNL). Completion of specific aims 1, 2 showed that the concentrations of the elements did not correlate with Gleason status. They also showed that B was unique in its variability. Aim 3 was accomplished in part, but imaging was impaired by ice crystal formation. We made progress in the 3rd year in both methodology to improve samples resolution and identifying subcellular B targets for B as a way around the resolution problem. These included: (1) Slam freezing samples to -196 deg C followed by vacuum dehydration <- 80 deg C to avoid ice crystal formation and identification of two potential subcellular proteins CD38 on the plasma membrane and the ryanodine receptor on the endoplasmic reticulum.
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 01, 2006
- Accession Number
- ADA463233
Entities
People
- Curtis D. Eckhert
Organizations
- University of California, Los Angeles