The Analysis of Cell Population Dynamics in Mammary Gland Development and Tumorigenesis

Abstract

The mammary gland is made up of two distinct epithelial layers that are derived from a common progenitor. The experiments in this proposal investigate the processes of mammary epithelial cell differentiation during development and tumorigenesis. Using FACS, mammary epithelial cell (MEC) populations from tumors and wildtype tissue were investigated for their outgrowth potential or tumorigenic capacity. We also developed new mouse models and imaging techniques that can be used to identify viable long-surviving cell populations and characterize these cells in real-time in vivo. We are also interested in studying how genes expressed in different mammary epithelial cell populations affect differentiation and tumorigenesis. For these experiments we have developed techniques for viral transduction and transplantation of primary MECs.

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Document Details

Document Type
Technical Report
Publication Date
Aug 01, 2006
Accession Number
ADA463667

Entities

People

  • Bryan Welm

Organizations

  • University of California, San Francisco

Tags

DTIC Thesaurus Topics

  • Biomedical Research
  • Breast Cancer
  • Cancer
  • Cell Line
  • Cell Physiological Processes
  • Cells
  • Culture Techniques
  • Dynamics
  • Epithelial Cells
  • Glands
  • Imaging Techniques
  • Infection
  • Mammary Glands
  • Neoplasms
  • Stem Cells
  • Transplants
  • Wound Infections

Fields of Study

  • Biology

Readers

  • Molecular Biology and Genetics

Technology Areas

  • Biotechnology