Role of Mitochondria in Prostate Cancer

Abstract

Most malignant cells are highly glycolytic and produce high levels of reactive oxygen species (ROS) compared to normal cells. Mitochondrial glycerophosphate dehydrogenase (mGPDH) participates in delivering reducing equivalents from this molecule into the electron transport chain thus sustaining of glycolysis. Here we investigate the role of mGPDH in maintaining an increased rate of glycolysis and evaluate glycerophosphate-dependent ROS generation in prostate cancer cell lines (LNCaP DU145 RC3 and CL1). Immunoblot Real Time RT-RCR polarographic and spectrophotometric analysis revealed that mGPDH abundance and activity was significantly elevated in prostate cancer cell lines when compared to normal prostate epithelial cell line PNT1A. Overall these data demonstrate that mGPDH is involved in maintaining a high rate of glycolysis and is an important site of electron leakage leading to ROS production in prostate cancer cells.

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Document Details

Document Type
Technical Report
Publication Date
Dec 01, 2006
Accession Number
ADA463829

Entities

People

  • Subir K. Chowdhury

Organizations

  • Hamilton Health Sciences

Tags

DTIC Thesaurus Topics

  • Adipose Tissue
  • Albumins
  • Cell Line
  • Cells
  • Chemistry
  • Connective Tissue
  • Cultured Cells
  • Diseases And Disorders
  • Epithelial Cells
  • Health Services
  • Metabolism
  • Mitochondria
  • Neoplasms
  • Oxidative Stress
  • Prostate Cancer
  • Sugar Phosphates
  • Tissues

Fields of Study

  • Biology
  • Chemistry

Readers

  • Aquatic Ecology
  • Immunology and Pathology
  • Oncology (Cancer Research).

Technology Areas

  • Microelectronics