A Dual-Action Armed Replicating Adenovirus for the Treatment of Osteoblastic Bone Metastases of Prostate Cancer
Abstract
In this Exploration - Hypothesis Development Award we hypothesize that the efficacy of a replicating adenovirus for the treatment of bone metastases of prostate cancer could be enhanced by arming it with the therapeutic protein sOPG-Fc which will block osteoclastic bone resorption and hence inhibit bone remodeling. Thus the objective of this proposal is to generate a dual-action armed replicating adenovirus which will both directly kill metastatic prostate cancer cells by oncolysis and will also secrete sOPG-Fc into the microenvironment of the bone thereby inhibiting osteoclastic bone resorption. To date we have constructed armed replication-selective adenoviruses expressing sOPG-Fc. We have confirmed that the sOPG-Fc gene is expressed in a similar temporal manner to the E3B genes which it replaced and that the remaining E3 genes continue to be expressed. We have also confirmed that expression of sOPG-Fc does not impair the selectivity or oncolytic potency of the armed replication-selective adenovirus.
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 01, 2006
- Accession Number
- ADA463916
Entities
People
- Joanne T. Douglas
Organizations
- University of Alabama