Knockout AR in Prostate
Abstract
Prostate cancer progresses from androgen-dependent to androgen-independent state. The androgen receptor (AR) is expressed throughout progression. We would like to understand the AR role in this progression. Using lox-Ore methodology we have generated mice in which AR function is abolished in the entire animal (ARKO) or tissue specific manner and generated mice with ARKO in prostate only or in different stages to be used to study prostate cancer (PCa) progresses. We aim to generate mice lacking AR in prostate epithelium. generate inducible ARKO mice to determine potential effects of androgen in AR absence on prostate growth/maintenance determine AR role in PCA development/progression by crossing ARKO mice with TRAMP mice to examine AR role in TRAMP induced PCa and permit determination of stages in PCa requiring AR function determine AR role in tumorigenicity of androgen- dependent/androgen-independent ARKO PCa cell lines. The effect of AR loss in these cells will be examined for ability to generate/promote tumors in mice. This year we generated mice with ARKO in the prostate epithelium and will be able to continue the other aims in the proposal in the coming years.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 2006
- Accession Number
- ADA464034
Entities
People
- Chawnshang Chang
Organizations
- University of Rochester