Ion Channel Conductance Measurements on a Silicon-Based Platform

Abstract

Conductance measurements of the transmembrane porin protein OmpF as a function of pH and bath concentration have been made with both a microfabricated silicon substrate device and a commercially available polystyrene aperture. Ion transport through the channel was simulated in atomic detail: the measured current was compared with theoretically calculated current, using a Brownian Dynamics kernel coupled to the Poisson equation by a P3M force field. The explicit protein structure and fixed charge distribution in the protein are calculated using the molecular dynamics code, GROMACS. Reasonable agreement is obtained in the simulated versus measured conductance over the range of experimental concentrations studied.

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Document Details

Document Type
Technical Report
Publication Date
Jan 01, 2006
Accession Number
ADA464359

Entities

People

  • Junyu Tang
  • L. Petrossian
  • M. Goyll
  • Marco Saraniti
  • R. S. Eisenberg
  • S. Aboud
  • S. J. Wilk
  • Stephen M. Goodnick
  • T. J. Thornton

Organizations

  • Arizona State University

Tags

Communities of Interest

  • Advanced Electronics
  • Biomedical

DTIC Thesaurus Topics

  • Amplifiers
  • Cell Membrane
  • Dielectric Permittivity
  • Electronics
  • High Voltage
  • Lipids
  • Measurement
  • Membrane Lipids
  • Membranes
  • Molecules
  • Platforms
  • Simulations
  • Small Molecules
  • Solid State Electronics
  • Standards
  • Universities
  • Voltage

Fields of Study

  • Physics

Readers

  • Computational Fluid Dynamics (CFD)
  • Molecular and Cellular Biochemistry
  • Quantum spin resonance or Electron Paramagnetic Resonance spectroscopy.