Sam68 and Breast Cancer

Abstract

Sam68 is a target for Src kinase. It is involved in HIV-1 RNA export. However, the role of Sam68 in cellular mRNA export is unknown. It has been well documented that patients have either lost or have mutations in BRCA1 or BRCA2 tumor suppressor genes are at high risk of developing breast cancers. Thus, the goal of this application is to investigate whether Sam68 is involved in the export of BRCA1 mRNA. To test this objective, we have generated stable Sam68 knockdown MCF7 cells. These reagents are critical to objectively test the role of Sam68 on BRCA1 expression. In this no-cost extension year, using MCF7 Sam68-RNAi stable cells, we expect to be able to examine how the reduced expression of Sam68 affects BRCA1 mRNA export. We will also identify the RNA export pathway for BRCA1 mRNA, proposed in Aim 2.

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Document Details

Document Type
Technical Report
Publication Date
Aug 01, 2006
Accession Number
ADA465244

Entities

People

  • Thipparthi R. Reddy

Organizations

  • Wayne State University

Tags

DTIC Thesaurus Topics

  • Abstracts
  • Antibodies
  • Biomedical Research
  • Breast Cancer
  • Cell Line
  • Cells
  • Department Of Defense
  • Electronic Mail
  • Information Operations
  • Maryland
  • Michigan
  • Neoplasms
  • Schematic Diagrams
  • Standards
  • Universities

Fields of Study

  • Biology

Readers

  • Molecular Biology and Genetics