Identification of Protein Kinases Required for NF2 Signaling

Abstract

The purpose of this concept award grant is to uncover potential drug targets for treatment of Neurofibromatosis type 2 (NF2). We planned a synthetic lethal screen, using RNAi technology to uncover protein kinases and phosphatases that are specifically required for the survival of NF2-null cells. We obtained and reformatted a murine siRNA library against all known protein kinases and phosphatases. We also obtained NF2flox/flox mouse embryo fibroblasts and used Ore recombinase to convert these to a NF24- genotype. We then tested a large number of transfection procedures to determine the most effective and least toxic method for delivery of siRNA into these cells, and optimized this procedure for a high throughput assay. Having carefully established the conditions for the screen, we are carrying out the main, most important experiment: the large scale screen for synthetic lethality.

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Document Details

Document Type
Technical Report
Publication Date
Dec 01, 2006
Accession Number
ADA465844

Entities

People

  • Jonathan Chernoff

Tags

DTIC Thesaurus Topics

  • Biological Sciences
  • Biomedical Research
  • Cell Physiological Processes
  • Cells
  • Culture Media
  • Department Of Defense
  • Genes
  • Genetic Phenomena
  • Genetic Structures
  • Genetics
  • Genotypes
  • Lethality
  • Neoplasms
  • Proteins
  • Recombinases
  • Survival
  • Transfection

Fields of Study

  • Biology

Readers

  • Molecular Biology and Genetics
  • Neurological Diseases/Conditions/Disorders
  • Systems Analysis and Design